Application of Response Surface Modeling and Chemometrics Methods for the Determination of Ofloxacin in Human Urine Using Dispersive Liquid-Liquid Microextraction Combined with Spectrofluorimetry

Application of Response Surface Modeling and Chemometrics Methods for the Determination of Ofloxacin in Human Urine Using Dispersive Liquid-Liquid Microextraction Combined with Spectrofluorimetry

Dispersive liquid-liquid microextraction (DLLME) and spectrofluorimetry, coupled with chemometrics methods, are proposed in this work for the pre-concentration and determination of ofloxacin concentration in spiked human urine. Chloroform and acetonitrile were selected as the extraction and dispersive solvents by the one-variable-at-a-time process. The Box-Behnken design was used to optimize th...

application of dispersive liquid–liquid microextraction combined with liquid chromatography for the determination of aldehydes in cigarette smoke

Modified ionic liquid cold-induced aggregation dispersive liquid-liquid microextraction combined with spectrofluorimetry for trace determination of ofloxacin in pharmaceutical and biological samples

BACKGROUND AND THE PURPOSE OF THE STUDY Ofloxacin is a quinolone synthetic antibiotic, which acts against resistant mutants of bacteria by inhibiting DNA gyrase. This antibacterial agent is widely used in the treatment of respiratory tract, urinary tract and tissue-based infections, which are caused by Gram-positive and Gram-negative bacteria. In this work, an efficient modified ionic liquid co...

Optimization of a Dispersive Liquid-Liquid Microextration Method for Analysis of Chlorpyrifos in Urine Using the Chemometrics Method

Aims: The use of simple, low cost, high -efficiency extraction methods for sample preparation in the forensic toxicology has been considered. Nowadays, the chemometrics technique can be used to determine the influencing factors on the response to optimize extraction methods. The aim of this study was to design and optimize a dispersive liquid-liquid microextraction (DLLME) method for extraction...

Evaluation of Response Surface Methodology in Dispersive Liquid-Liquid Microextraction for Lead Determination Using Ionic Liquids

This paper describes a dispersive liquid–liquid microextraction (DLLME) procedure using room temperature ionic liquids (RTILs) coupled with flame atomic absorption spectrometry detection with microsample introduction system capable of quantifying trace amounts of lead. In the proposed approach, ammonium pyrrolidine dithiocarbamate (APDC) was used as a chelating agent and 1-hexyl-3-methylimmidaz...